Product Introduction
Total SOD Activity Detection Kit (WST-8 method) is a kit based on the colorimetric reaction of WST-8, which detects the activity of SOD, i.e. superoxide dismutase, in cells, tissues or other samples by colorimetry.
Superoxide dismutase (SOD) can catalyze the dismutation of superoxide anions to generate hydrogen peroxide (H2 O2 ) and oxygen (O2 ), and is an important antioxidant enzyme in organisms. There are currently a variety of SOD activity assays, among which the NBT (nitroblue tetrazolium) method is widely used due to its ease of use. However, the formazan dye produced by the NBT method has poor water solubility and is easily interacted with the reduced xanthine oxidase, resulting in an inhibition rate that is less than 100%, which affects the sensitivity and accuracy of the assay. The cytochrome C method is also a commonly used method for detecting SOD activity, but cytochrome C has high oxidative activity and is easily interfered by reducing agents in the sample. In addition, this method requires continuous measurement of absorbance values, and has a relatively low sensitivity for SOD detection, and is not suitable for the detection of large quantities of samples. The WST-1 method is similar to the WST-8 method, and is more advanced than the above methods. This kit adopts the WST-8 method, which has better stability and higher sensitivity among the current SOD determination methods, and can detect SOD as low as 0.5U/ml.
Product Features
Methodological advantages:
1. The reaction product of WST-8 is a stable water-soluble product. The SOD enzyme activity can be measured by absorbance detection at a single time point, which is suitable for high-throughput screening research. When measuring SOD enzyme activity with the WST-8 method, the maximum inhibition percentage can be close to 100 %, and can not be interfered by some common interference factors, so that the detection effect is significantly improved than some other common methods.
2. The performance of this kit is better than the total SOD activity detection kit (NBT method) of similar products.
3. The purpose of this product is the same as the total SOD activity detection kit (NBT method) of similar products. The change in absorbance caused by the same amount of SOD is significantly greater than that of the NBT method, and the linear range is wider.
Convenience: The SOD sample preparation solution provided in this kit can directly lyse cells without homogenization, making the operation more convenient.
Principle
WST-8 can react with superoxide anions (O2-) produced by xanthine oxidase (XO) to produce water-soluble formazan dye. Since SOD can catalyze the dismutation of superoxide anions, this reaction step can be inhibited by SOD. Therefore, the activity of SOD is negatively correlated with the amount of formazan dye generated, so the enzyme activity of SOD can be calculated by colorimetric analysis of the WST-8 product. The schematic diagram of this kit is shown as below
Components
Serial number | Components | Size(100T) | Storage |
Reagent 1 | SOD Sample Preparation Solution | 50ml | -20 ℃ |
Reagent 2 | SOD Assay Buffer | 50ml | -20 ℃ |
Reagent 3 | WST-8 | 800µl | -20 ℃ , avoid light |
Reagent 4 | Enzyme Solution | 100µl | -20 ℃ |
Reagent 5 | Reaction Start Solution (16X) | 150µl | -20 ℃ |
Microplate(96 wells) | 1 plate | RT | |
Plate Sealer | 2 pieces | RT |
Storage
The unopened kit can be stored at -20 ℃ for 12 months .
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
