Product Introduction

Reactive oxygen species (ROS) mainly include hydroxyl radicals, superoxide radicals and hydrogen peroxide. Reactive oxygen species can cause oxidative damage to intracellular lipids, proteins and DNA, induce oxidative stress, and then lead to various tumors, atherosclerosis, rheumatoid arthritis, diabetes, liver damage, and central nervous system diseases.

There are many kinds of antioxidants in the body, including antioxidant macromolecules, antioxidant small molecules and enzymes, which can remove various reactive oxygen species produced in the body to prevent the generation of oxidative stress induced by reactive oxygen species. The total level of various antioxidant macromolecules, antioxidant small molecules and enzymes in a system reflects the total antioxidant capacity in the system. Therefore, it is of great biological significance to measure the total antioxidant capacity in various body fluids such as plasma, serum, urine, saliva, and lysates of cells or tissues.

The detection of the total antioxidant capacity of plant or Chinese herbal extracts, or various antioxidant solutions can be used to detect the strength of the antioxidant capacity of various solutions, and can be used to screen drugs with strong antioxidant capacity.

Product Features

This kit is convenient and quick. The absorbance measurement can be performed 2-6 minutes after adding the sample to be tested. Usually 10-20 samples can be tested in more than ten minutes.

Principle    

This kit uses ABTS (2,2'-hydrazinobis[3-ethylbenzothiazole-6-sulfonic acid]) as a colorimetric agent, which can accurately determine the total antioxidant capacity in the sample. ABTS is oxidized to green ABTS+ in the presence of an appropriate oxidant. The production of ABTS+ will be inhibited in the presence of antioxidants. The total antioxidant capacity of the sample can be determined and calculated by measuring the absorbance of ABTS+ at 734nm or 405nm. Trolox is an analog of vitamin E with an antioxidant capacity similar to that of vitamin E. It is used as a reference for the total antioxidant capacity of other antioxidants. For example, the total antioxidant capacity of Trolox is 1. Under the same concentration, the antioxidant capacity of other substances is expressed as a multiple of their antioxidant capacity compared to Trolox.

Components

Storage

The unopened kit can be stored at -20℃ for 12 months.

Notes 

This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.