Product Introduction
Glutathione Peroxidase (GSH-Px) Activity Assay Kit (NADPH Method) is a commonly used metabolism assay kit for detecting the activity of Glutathione Peroxidase (GPx). GPx is an important antioxidant enzyme that can eliminate harmful substances such as hydrogen peroxide in the human body, protect cells from free radical damage, and maintain the stability of the intracellular environment. Lipids in cells are prone to react with free radicals to produce lipid peroxides. Glutathione Peroxidase can use reduced glutathione (GSH) to reduce lipid peroxides, thereby eliminating the toxic effects of free radicals. Glutathione Peroxidase is distributed in almost all tissues. In some pathological conditions, the activity of Glutathione Peroxidase will be significantly up- or down-regulated.
Product Features
1. The organic peroxide reagent (t-Bu-OOH) provided in this kit does not react with GSH in the absence of Glutathione Peroxidase, nor is it catalyzed and decomposed by
cellular catalase. Therefore, it can relatively specifically detect the activity of Glutathione Peroxidase.
2. Since t-Bu-OOH cannot be catalyzed by selenium-free Glutathione Peroxidase, this kit can relatively specifically quantify the most common selenium containing Glutathione Peroxidase.
Principle
GPx catalyzes the reaction of reduced glutathione (GSH) with peroxide reagent (R-OOH) to convert it into oxidized glutathione (GSSG). Glutathione reductase (GR) can use NADPH to catalyze GSSG to produce GSH. In the above reaction, Glutathione Peroxidase is the rate-limiting step of the entire reaction system, so the decrease in NADPH is linearly related to the activity of Glutathione Peroxidase. Therefore, by detecting the change in A340, the amount of NADPH decrease, the activity level of GPx can be calculated.
Components
Components | Size(100T) | Storage |
Sample Homogenate | 100ml | -20℃ |
Glutathione Peroxidase Detection Buffer | 50ml | -20℃ |
Glutathione Reductase | 100μl | -20℃ |
NADPH | 11.5mg | Store at -20℃, after dissolving NADPH, aliquot and store at -70ºC, can be stored at 4ºC for one day, NADPH will degrade by more than 10% after one week at -20ºC |
Reduced Glutathione (GSH) | 10mg | Store at -20℃, GSH should be prepared as a solution, aliquot and store at -20ºC |
Peroxide Reagent(t-Bu-OOH) | 200μl | -20℃ |
Microplate(96 wells) | 1 plate | RT |
Plate Sealer | 2 pieces | RT |
Storage
Unopened kits can be stored at -20℃ for 12 months.
Notes
This product is intended for scientific research use only by professionals and must not be used for clinical diagnosis or treatment, in food or drugs, or stored in ordinary residences.
