Recombinant Human EDF1 (C-6His)-Target Proteins-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

Name	Recombinant Human EDF1 (C-6His)
Purity	Greater than 95% as determined by reducing SDS-PAGE
Endotoxin level	<1 EU/µg as determined by LAL test.
Construction	Recombinant Human Endothelial Differentiation-Related Factor 1 is produced by our E.coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.
Accession #	O60869
Host	E.coli
Species	Human
Predicted Molecular Mass	17.4 KDa
Buffer	Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
Form	Lyophilized
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Stability&Storage	Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Alternative Names

Endothelial Differentiation-Related Factor 1; EDF-1; Multiprotein-Bridging Factor 1; MBF1; EDF1

Background

Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.

Note

For Research Use Only , Not for Diagnostic Use.