Summary

Performance

Immunogen

Application

Background

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing resultsdomain:The leucine-rich repeats and the NTF2-domain are essential for the export of mRNA from the nucleus.,domain:The minimal CTE binding domain consists of an RNP-type RNA binding domain (RBD) and leucine-rich repeats.,domain:The nucleoporin binding domain consists of a NTF2-like domain and a TAP domain (also called UBA-like domain). The NTF2 domain heterodimerizes with NXT1 and NXT2. The formation of NXF1/NXT1 heterodimers is required for NXF1-mediated nuclear mRNA export. The TAP domain mediates direct interactions with nucleoporin-FG-repeats and is necessary and sufficient for localization of NXF1 to the nuclear rim. The conserved loop 594-NWD-596 of the UBA domain has a critical role in the interaction with nucleoporins.,function:Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm.,miscellaneous:The RNA-binding domain is a non-canonical RNP-type domain.,similarity:Belongs to the NXF family.,similarity:Contains 1 NTF2 domain.,similarity:Contains 1 RRM (RNA recognition motif) domain.,similarity:Contains 1 TAP-C domain.,similarity:Contains 4 LRR (leucine-rich) repeats.,subcellular location:Localized predominantly in the nucleoplasm and at both the nucleoplasmic and cytoplasmic faces of the nuclear pore complex. Shuttles between the nucleus and the cytoplasm.,subunit:Interacts with NXT1, NXT2, E1B-AP5, RAE1, THOC4 and with several nucleoporins. Is part of the exon junction complex (EJC) containing NCBP1, NCBP2, RNPS1, RBM8A, SRRM1, NXF1, RENT1, RENT2, RENT3A, RENT3B and THOC4. Found in a mRNA complex with RENT3A and RENT3B. Interacts with Saimiriine herpesvirus 2 TIP protein. Interacts with NUPL2.,tissue specificity:Expressed ubiquitously.,

Research Area