Basic Information
Product Name | Hoechst 33258 Nuclear Staining Solution (Ready-to-use) |
Size | 10mL |
Storage conditions | -20 ℃, protected from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Molecular Structure
Hoechst 33258 excitation/emission wavelength : 346~352/460~461 nm
Product Introduction
Hoechst 33258, also known as bis Benzimide H 33258 or HOE 33258, is a non-intercalating bright blue fluorescent dye. The fluorescence of the dye is weak in solution, but it becomes bright when it binds to the minor groove of DNA in AT-rich regions in living cells. Therefore, these dyes are also referred to as DNA probes. Due to the low background, there is no need for a washing step after staining, and the staining is very stable, non-toxic to live cells, and can last for several days or even longer after binding to DNA. Compared with Hoechst 33342, Hoechst 33258 has higher solubility in water, but both dyes have high cell membrane permeability and are widely used for apoptosis detection. After staining, they can be observed with a fluorescence microscope or detected by flow cytometry.
Note: This reagent is for scientific research use only!
