Basic Information
Product Name | Enhanced DiO Membrane Probe (Green) |
Size | 5mg |
Storage conditions | Store at 4 °C, away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Enhanced DiO excitation/emission wavelength：484/501nm
Product Introduction
DiO is a green fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
Reagent preparation
（1）Stock Solution Preparation:
The stock solution should be prepared using DMSO or EtOH, with a concentration of 1–5 mM.
Note: Unused stock solution should be aliquoted and stored at -20°C to avoid repeated freeze-thaw cycles.
（2）Working Solution Preparation:
Dilute the stock solution with an appropriate buffer (such as serum-free culture medium, HBSS, or PBS) to prepare a working solution with a concentration of 1–5 μM.
Note: The final concentration of the working solution is recommended to be optimized based on different cell lines and experimental systems. It is suggested to start exploring the optimal concentration within a range of 10 times the recommended concentration.
Note: This reagent is for scientific research use only!
