Basic Information
Product Name | DiR Membrane Probe (Near-Infrared) |
Size | 5 mg |
Storage | Store at -20 ℃, protected from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Molecular Structure
DiR excitation/emission wavelength: 748/780nm
Product Introduction
DiR is a near IR fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
Reagent preparation
（1）Preparation of stock solution: The stock solution is prepared with anhydrous DMSO or EtOH at a concentration of 1~5 mM.
Note: Unused stock solution should be stored in aliquots at -20℃ to avoid repeated freezing and thawing.
（2）Preparation of working solution: Dilute the stock solution with an appropriate buffer (e.g. serum-free medium, HBSS or PBS) to prepare a working solution with a concentration of 1~5 μM.
Note: The final concentration of the working solution is recommended to be optimized according to different cell lines and experimental systems. It is recommended to start exploring the optimal concentration within a range of 10 times the recommended concentration.
Note: This reagent is for scientific research use only!
