Basic Information
Product Name | DiO Membrane Probe (Green) |
Size | 10mg |
Storage | Store at 4 °C, away from light |
Shipping | Shipped with ice pack |
Validity | 12 months |
Molecular Structure
DiO excitation/emission wavelength: 484/501nm
Product Introduction
DiO is a green fluorescent, lipophilic carbocyanine and widely used as a lipophilic tracer. It is weakly fluorescent in water but highly fluorescent and quite photostable when incorporated into membranes. It has an extremely high extinction coefficient and short excited-state lifetimes (~1 nanosecond) in lipid environments. Once applied to cells, the dye diffuses laterally within the plasma membrane.
Reagent preparation
Staining solution preparation
(1) Preparation of stock solution: The stock solution is prepared with DMSO, anhydrous DMF or EtOH , with a concentration of 1-5 mM . The solubility of DiO in anhydrous DMSO and anhydrous DMF is higher than that in EtOH.
Note: a. Unused storage solution should be stored in aliquots at -20°C to avoid repeated freezing and thawing;
b. If the solution is difficult to dissolve, it can be heated appropriately and ultrasonicated to promote dissolution.
(2) Preparation of working solution: Dilute the stock solution with a suitable buffer (e.g. serum-free culture medium, HBSS or PBS) to prepare a working solution with a concentration of 1-30 μM. The most commonly used working solution concentration is 5-10 μM.
Note: The final concentration of the working solution is recommended to be optimized according to different cell lines and experimental systems. It is recommended to start exploring the optimal concentration within 10 times the recommended concentration.
Note: This reagent is for scientific research use only!
