Summary
| Production Name | TATA Box Binding Protein (9D1) Mouse Monoclonal Antibody |
| Description | Mouse Monoclonal Antibody |
| Host | Mouse |
| Application | WB |
| Reactivity | Human,Rat,Mouse |
Performance
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | IgG1 |
| Clonality | Monoclonal Antibody |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Purification | Affinity Purification |
Immunogen
| Gene Name | TBP |
| Alternative Names | TBP; GTF2D1; TF2D; TFIID; TATA-box-binding protein; TATA sequence-binding protein; TATA-binding factor; TATA-box factor; Transcription initiation factor TFIID TBP subunit |
| Gene ID | 6908 |
| SwissProt ID | P20226 |
Application
| Dilution Ratio | WB: 1:500-1:1000 |
| Molecular Weight | Calculated MW: 38 kDa; Observed MW: 35-45 kDa |
Background
As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed.
Research Area
Epigenetics and Nuclear Signaling
