Summary
| Production Name | GRP78 BiP (9E4) Mouse Monoclonal Antibody |
| Description | Mouse Monoclonal Antibody |
| Host | Mouse |
| Application | WB,ICC/IF |
| Reactivity | Human,Rat |
Performance
| Conjugation | Unconjugated |
| Modification | Unmodified |
| Isotype | IgG1 |
| Clonality | Monoclonal Antibody |
| Form | Liquid |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3. |
| Purification | Affinity Purification |
Immunogen
| Gene Name | HSPA5 |
| Alternative Names | HSPA5; GRP78; 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP |
| Gene ID | 3309 |
| SwissProt ID | P11021 |
Application
| Dilution Ratio | WB: 1:500-1:1000 IF: 1:50-1:200 |
| Molecular Weight | Calculated MW: 72 kDa; Observed MW: 78 kDa |
Background
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) (PubMed 8020977) pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER).
Research Area
Tags & Cell Markers
