Recombinant Cynomolgus TIGIT (C-Fc)-Target Proteins-EnkiLife-Recombinant Proteins,Antibodies,Kits,Cell Biology

Name	Recombinant Cynomolgus TIGIT (C-Fc)
Purity	Greater than 95% as determined by reducing SDS-PAGE
Endotoxin level	<1 EU/µg as determined by LAL test.
Construction	Recombinant Cynomolgus T cell Immunoreceptor With Ig And ITIM Domains is produced by our Mammalian expression system and the target gene encoding Met89-Pro209 is expressed with a human IgG1 Fc tag at the C-terminus.
Accession #	G7NXM4
Host	Human Cells
Species	Cynomolgus
Predicted Molecular Mass	40.6 KDa
Buffer	Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Form	Lyophilized
Shipping	The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.
Stability&Storage	Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
Reconstitution	Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Alternative Names

T-cell immunoreceptor with Ig and ITIM domains; VSIG9; VSTM3; TIGIT; V-set and transmembrane domain-containing protein 3; V-set and immunoglobulin domain-containing protein 9

Background

T cell immunoreceptor with Ig and ITIM domains (TIGIT), also called VSIG9 and Vstm3, is a member of the CD28 family within the Ig superfamily of proteins. TIGIT contains an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM), and is expressed on regulatory, memory, activated T cells and NK cells. TIGIT binds to CD155(PVR) that appear on dendritic cells (DC), macrophages and endothelium with high affinity, and CD112(PVRL2) with lower affinity, but not CD113 (PVRL3). TIGIT-Fc fusion protein could interact with PVR on DC and enhance the secretion of IL-10, but inhibit the macrophage activation. Mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges, while knockdown of TIGIT with siRNA in human memory T cells did not affect T cell responses.

Note

For Research Use Only , Not for Diagnostic Use.