Recombinant Human ASF1A (C-6His, N-T7 tag)

Recombinant Human ASF1A (C-6His, N-T7 tag)

Size1:10μg price1:$168
Size2:50μg price2:$465
Size3:500μg price3:$2350 SKU: PEH0794 Category: Target Proteins Tags: ,

Datasheet

Name

Recombinant Human ASF1A (C-6His, N-T7 tag)

Purity

Greater than 95% as determined by reducing SDS-PAGE

Endotoxin level

<1 EU/µg as determined by LAL test.

Construction

Recombinant Human Histone Chaperone ASF1A is produced by our E.coli expression system and the target gene encoding Met1-Met204 is expressed with a T7 tag at the N-terminus, 6His tag at the C-terminus.

Accession #

Q9Y294

Host

E.coli

Species

Human

Predicted Molecular Mass

25.4 KDa

Buffer

Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 1mM DTT, 150mM NaCl, pH 8.0.

Form

Lyophilized

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Stability&Storage

Store at ≤-70°C, stable for 6 months after receipt.Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Reconstitution

Always centrifuge tubes before opening.Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

 

 

 

Alternative Names

Histone Chaperone ASF1A; Anti-Silencing Function Protein 1 Homolog A; hAsf1; hAsf1a; CCG1-Interacting Factor A; CIA; hCIA; ASF1A

 

Background

Human Histone Chaperone ASF1A (ASF1A) belongs to the H3/H4 family of histone chaperone proteins. ASF1A is ubiquitously expressed in many cells and tissues, interacting with histones H3 and H4. ASF1A cooperates with Chromatin Assembly Factor 1 to promote replication-dependent chromatin assembly and with HIRA to promote replication-independent chromatin assembly. In addition, ASF1A is necessary for the formation of senescence-associated heterochromatin foci (SAHF) and efficient senescence-associated cell cycle exit.

 

Note

For Research Use Only , Not for Diagnostic Use.

 

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