Product Information
This product is a novel and stable siRNA-specific transfection reagent. It has a strong ability to condense RNA, enabling the efficient and rapid transfection of RNA into eukaryotic cells without degradation by nucleases. Compared to other transfection reagents, it offers several advantages: low toxicity, high stability, strong serum tolerance, simple and easy-to-follow transfection procedures, and excellent reproducibility.
Optimization of siRNA Transfection
To achieve optimal transfection results, the transfection process can be optimized by adjusting the following parameters: cell density, siRNA concentration, and transfection reagent concentration. When conducting optimization experiments, ensure that the cell confluence is above 60%, and the ratio of transfection reagent (µL) to siRNA (pmol) can be flexibly adjusted between 0.02:1 and 0.15:1 to find the most suitable transfection conditions for the target cell line.
Table.1 Reference Table for Transfection Reagent and siRNA Usage in Different Culture Plates
Culture Plate | Single Well Area (cm²) | Seeded Cell Number (cells) | Final Volume of Opti-MEM Dilution (µL) | Transfection Reagent Usage (µL) | siRNA Usage (µg) |
96-well | 0.3cm2 | 200µL | 10µL | 0.5µL | 7.5 pmol |
24-well | 2.0cm2 | 500µL | 20µL | 1.0µL | 15 pmol |
12-well | 4.0cm2 | 1mL | 40µL | 2.0µL | 30 pmol |
6-well | 10.0cm2 | 2mL | 100µL | 4.0µL | 60 pmol |
Precautions
1.Before using this product, please read this specification sheet carefully and strictly follow the recommended procedures to ensure optimal transfection results.
2.This product is intended for research use only and should not be used for clinical diagnosis or treatment or any other purposes.
