Product Introduction

This 3-Color Multiple Fluorescent Staining Kit (mIHC) is suitable for double immunofluorescence staining of tissue samples, including paraffin sections, frozen sections, cell slides, cell smears, and organoids. It is particularly suitable for double fluorescent immunolabeling using primary antibodies from the same source, but can also be used with antibodies from different sources. The primary principle of TSA is based on tyramide signal amplification (TSA ) , hereinafter referred to as TSA technology. TSA technology utilizes the peroxidase reaction of tyramide (wherein a fluorescently labeled tyramide forms a covalent binding site under HRP-catalyzed H₂O₂). This generates a large number of enzymatic reactions, which then react with surrounding protein residues (including tryptophan, histidine, and tyrosine residues), forming a large amount of fluorescent pigment deposited at the antigen-antibody binding site, thereby amplifying the signal.

Product Information

Product form	liquid
Product Specifications	20T , 50T , 100T
Storage conditions	2-8 ℃
Conditions of Transport	Low temperature
Validity period	12 months

Components	Sizes			Dilutions
Components	20T	50T	100T	Dilutions
TSA- CY3	10μL	25μL	50μL	1:200
TSA-FITC	10μL	25μL	50μL	1:200
TSA diluent	4mL	10mL	20mL	Ready-to-use
3% H₂O₂	2mL	5mL	10mL	Ready-to-use
Blocking solution	4mL	10mL	20mL	Ready-to-use
Primary antibody diluent	4mL	10mL	20mL	Ready-to-use
Antibody elution reagent	2mL	5mL	10mL	Ready-to-use
HRP- goat anti-rabbit/mouse IgG	4mL	10mL	20mL	Ready-to-use
DAPI staining	2mL	5mL	10mL	Ready-to-use
Anti-fluorescence fading mounting medium	2mL	5mL	10mL	Ready-to-use

3-Color Multiple Fluorescent Staining Kit (mIHC)

3-Color Multiple Fluorescent Staining Kit (mIHC)

Product Introduction

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