Protocols

Western blotting is a widely used immunological method for detecting specific proteins in complex mixtures. It combines the separation power of gel electrophoresis with the specificity of antibodies. The process begins with protein extraction from cells or tissues, followed by separation by SDS-PAGE based on molecular weight. Proteins are then transferred to a membrane (typically nitrocellulose or PVDF) and detected using specific antibodies. The primary antibody binds to the target protein, while the secondary antibody, conjugated to a reporter enzyme, enables visualization through chemiluminescent, fluorescent, or colorimetric detection.

Western Blotting (also called immunoblotting) is a technique used for analysis of individual proteins in a protein mixture (e.g. a cell lysate). In Western blotting (immunoblotting) the protein mixture is applied to a gel electrophoresis in a carrier matrix (SDS-PAGE, native PAGE, isoelectric focusing, 2D gel electrophoresis, etc.) to sort the proteins by size, charge, or other differences in individual protein bands.